Digital Fluorometer Manufacturer,Supplier and Exporter in India
Product Code : EL-RE-2209
Jlab Export is a leading Digital Fluorometer Manufacturer,and suppliers in India
Supplied with a set of five filters and five test tubes, this digital fluorometer measures the light a fluorescent sample gives out when a tungsten halogen lamp excites it. It covers the visible range and reads on a 3-digit LED display. Quinine sulphate at 1 ppm in 0.1 N sulphuric acid gives full-scale deflection, and sensitivity is adjustable in 4 ranges.
Specifications
| Display | 3-digit bright red seven-segment LED |
| Excitation source | 12 V, 50 W tungsten halogen lamp |
| Sensitivity | Full-scale deflection with 1 ppm quinine sulphate in 0.1 N sulphuric acid (primary wavelength 360 nm) |
| Sensitivity ranges | Adjustable in 4 ranges |
| Filters | 2 primary (excitation) and 3 secondary (emission) filters, mounted on holders |
| Detector | Wide-range photodiode/cell |
| Sample tube | 15 mm (D) x 80 mm (H), minimum sample 4 ml |
| Power supply | 230 V ±10%, 50 Hz AC |
| Size and weight | 260 x 335 x 160 mm, 4.5 kg (approx.) |
| Standard accessories | Set of 5 primary and secondary filters, set of 5 test tubes |
| Minimum order quantity | 1 unit |
| Dispatch time | Usually 7–10 working days |
| Manufacturer | JLab (Jain Laboratory Instruments Pvt Ltd), Ambala, India |
Fluorimetry Practicals for Pharmacy Students
Pharmacy and chemistry students make a series of quinine sulphate standards in dilute acid. They pipette the stock with Bulb Pipettes, Class B Soda Glass, One Mark JLab and make up each standard in Volumetric Flasks, Class A Borosilicate Glass Plastic Stopper JLab. After setting full scale with the strongest standard, they plot reading against concentration and read an unknown, such as diluted tonic water, from the line.
A second practical shows quenching. Adding a little Sodium Chloride solution to a quinine standard lowers the reading, because chloride ions quench quinine fluorescence. This is also why the standards are made in Sulphuric Acid Dilute and not in hydrochloric acid.
Frequently Asked Questions
How do I choose the primary and secondary filters?
The primary filter passes the exciting light, and the secondary filter passes only the emitted light, which has a longer wavelength. Pick a pair that matches the excitation and emission bands of your compound. For quinine, the primary filter is chosen near its 360 nm excitation.
How do I zero and set the instrument?
Read a blank of the solvent alone and set the display to zero. Insert the strongest standard and set it to full scale on a suitable sensitivity range. Then read the other standards and the samples without changing any setting.
Why is quinine read in 0.1 N sulphuric acid?
Quinine fluoresces strongly in dilute sulphuric acid, and the sensitivity of this instrument is stated with 1 ppm quinine sulphate in 0.1 N acid. Hydrochloric acid is avoided because its chloride ions quench the fluorescence and lower every reading.
Why do readings level off at higher concentrations?
A strong solution absorbs much of the exciting light near the tube wall and reabsorbs some emitted light, so the plot bends. Dilute the sample until it falls on the straight part of your standard curve.
What sample volume and tube does it take?
It takes test tubes 15 mm in diameter and 80 mm high, with at least 4 ml of sample. Five tubes come as standard accessories. Hold tubes by the top and wipe them before reading, since fingerprints and dust scatter light.
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Last updated: 6 November 2026
